Abstract
Dibenzothiophene (DBT) was oxidized with supplement of energy source such as lactate or glycerol by resting cells of Pseudomonas jianii. Necessity of the supplement was in agreement with the phenomenon of co-metabolism. DBT oxidizing enzymes were induced by benzene ring having no side chain such as DBT, naphthalene, and anthracene. Supplemental substance, co-substrate, was used as energy source for synthesis of the enzymes. Induction of the enzymes was significantly repressed by presence of glucose, and the repression could be overcome by addition of cyclic-3', 5'-adenosine monophosphate (cAMP).
