Isolation of Tryptic Peptides from the Ile Chain of Ricin D and the Sequences of Some Tryptic Peptides Including N- and C-Terminal Peptides

Abstract

Twenty tryptic peptides were isolated from the performic acid-oxidized Ile chain of ricin D by Dowex 1×2 column chromatography followed by paper chromatography. The amino acids contained in these peptides accounted for 218 out of 266 residues in the whole protein. The amino acid sequences of nine peptides were determined by manual liquid phase or automatic solid phase Edman degradation, and N- and C-terminal sequences of the Ile chain of ricin D were established to be NH₂-Ile-Phe-Pro-Lys-Gln-Tyr-Pro-Ile-Ile- and Cys-Ala-Pro-Pro-Pro-Ser-Ser-Gln-Phe, respectively.