Effect of Materials Released from Myofibrils by CAF (Ca²⁺-activated Factor) on Z-Disk Reconstitution

Abstract

To investigate the constituents of Z-disk, reconstitution of Z-disk by incubating some proteins released from myofibrils by CAF (Ca²⁺-activated factor) with Z-disk-extracted fiber bundles was carried out and examined with electron microscope. The materials released from myofibrils by CAF have been bound in Z-disk region, and Z-disk extracted from myo-fibrils with a low ionic strength solution has been reconstituted. On the other hand, Z-disk removed from myofibrils by CAF has not been reconstituted by the same way.