抄録
Improvements were found in the inosine productivity of Brevibacterium ammoniagenes KY 13714, which is an adenine leaky and 6-mercaptoguanine resistant mutant. A highly productive mutant, KY 13761, was selected after the addition of 6-methylthiopurine resistance and guanine requiring character to KY 13714 and after repeating single colony isolation.
Culture conditions for the practical production of inosine were investigated using KY 13761. It was found that the concentrations of phosphate, magnesium, and adenine were important. Carbon sources and natural nutrients also showed profound effects on inosine accumulation. Especially, effective was the feeding of inverted molasses and urea for pro-duction of inosine. Under optimal conditions, 31 mg of inosine per ml was accumulated after 42 hr cultivation in a 5 liter jar fermenter at 32°C. A growth-associated type of accumu-lation was confirmed in inosine production with KY 13761.
