Studies on Trypsin Covalently Bound to RNA
1978 年 42 巻 4 号 p. 799-801
詳細
1978 年 42 巻 4 号 p. 799-801
Bovine trypsin (EC 3.4.4.4) was bound to RNA in the presence of water-soluble carbo-diimide [1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide], and the enzymic properties of this complex were compared with those of free trypsin. RNA bound trypsin does not lose its activity in the presence of 0.5% SDS, and this is consistent with the fact that the intensity of the fluorescence spectrum of this complex does not change when SDS is added. The RNA-enzyme complex seems suitable for studies on the conformation of immobilized enzyme.
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