抄録
Bacterial strains capable of assimilating methanol as the sole source of carbon and energy have been isolated. These bacteria produced phenazine methosulfate-dependent methanol dehydrogenase (primary alcohol dehydrogenase, EC 1.1.99.8). The enzyme activity was detected in electrophoresis on polyacrylamide gels by activity staining technique. The dehydro-genase can be grouped to at least three types based on their mobility in electrophoresis: (1) enzyme which migrates toward the anode slowly, (2) enzyme which remains at the top of the gel at pH 9.3 and (3) enzyme which migrates to the anode at faster mobility. The three bacteria were selected as producers of the respective three types of dehydrogenase. They were facultative methanol-utilizing bacteria and two of them were identified, one as Pseudomonas sp. No. 2941 and the other as Pseudomonas sp. S25. Most isolated bacteria produced the first type of enzyme and several strains produced the second type of enzyme. Strain. S50 was the only strain which produced the third type of enzyme. These groupings of methanol dehydrogenase may contribute to the classification of methylotrophic bacteria.
