A Differential Determination Procedure for Spermine and Spermidine with Beef Plasma Amine Oxidase

Abstract

An enzymatic procedure for the differential determination of polyamines, spermine and spermidine, has been established using beef plasma amine oxidase. This method was specific for these polyamines and required only one reaction system. Small amounts of polyamines (10μM to 80μM of spermine and 10μM to 100μM of spermidine) were assayed by solving two simultaneous equations obtained from the rate assay method and the end point assay method. The calculated values were in good agreement with those obtained by other method.