Abstract
Enzymatic synthesis of oligosaccharide through the transglucosylation reaction of bre-wer's yeast α-glucosidase was attempted in the reaction system containing only sucrose as sub-strate. As the transglucosylation product, a new nonreducing trisaccharide was chromato-graphically isolated in crystalline form (monohydrate), giving mp 139_??_142°C and [α]22D+120 (in water). The trisaccharide was confirmed to be 6F-α-D-glucosyl-sucrose, that is, O-α-D-glucopyranosyl-(1→6)-;O-β-D-fructofuranosyl-(2→1)-O-α-D-glucopyranoside, of which undeca-acetate gave mp 148°C and [α]22D+119° (in chloroform), and this sugar was named “isomelezitose.”
