N-Acetylglutamate-acetylorinithine Acetyltransferase-Deficient Arginine Auxotroph of Corynebacterium glutamicum

Abstract

An N-acetylglutamate-acetylornithine acetyltransferase-deficient arginine-requiring mutant AA-1, was derived from an L-arginine producer of Corynebacterium glutamicum. It accumulated a large amount (30mg per ml) of L-glutamic acid and a small amount (1.2mg per ml) of N^α-acetylornithine, an intermediate of arginine biosynthesis, in the culture medium.
The production of N^α-acetylornithine by AA-1 was not affected by the concentration of L-arginine in the medium, whereas that of L-glutamic acid was inhibited by a high concentration of L-arginine in the medium containing excess biotin.