Nonlocalized Membrane Growth Studied by the Density Labeling Technique

Abstract

A mixture of heavy and light membranes could be fragmented in the presence of 20% sucrose with only limited hybrid formation. The limiting size to which membranes can be fragmented without hybridization was approximately 500 Å. The membranes from the density shift or from both the density and temperature shifts gave no indication for distinctly light or heavy membrane patches within the limit of detection.