Immobilization of Biocatalysts by Prepolymer Methods. Adenylate Kinase Activity of Immobilized Yeast Mitochondria

抄録

Yeast mitochondria were immobilized easily and efficiently by entrapment with a photo-crosslinkable resin prepolymer or a urethane prepolymer. The immobilized gel-entrapped organelles showed a relatively high activity of adenylate kinase, converting ADP to ATP and AMP. Immobilization greatly enhanced the stability of mitochondrial adenylate kinase during storage at 4°C or incubation at 30°C. The immobilized mitochondria retained almost the original enzyme activity even after 19 batches of the reaction (total operational period, 380min). These results indicate the utility of the immobilized mitochondria as an immobilized preparation of adenylate kinase.