NAD-Dependent Maltose Dehydrogenase and NAD-Dependent D-Glucose Dehydrogenase of Alkalophilic Corynebacterium sp. No. 150-1

Abstract

An alkalophilic bacterium, strain No. 150-1, which had NAD-dependent sugar dehydrogenase activities on maltose (NAD-MalDH) and D-glucose (NAD-GlcDH), was isolated from soil. This microorganism was identified to be in a strain of the genus Corynebacterium. The bacterium grew to similar degrees at Na₂CO₃ concentrations from 0 to 0.5%. NAD-MalDH and NAD-GlcDH were not inducible types. Soybean casein was the most effective nitrogen source for enzyme production. Activity staining of these two dehydrogense on polyacrylamide gel showed that these activities were derived from two different proteins.The cell free extract did not contain NADP-dependent maltose dehydrogenase.