Determination of Glucose by a Modification of Somogyi-Nelson Method

抄録

Nelson's arsenomolybdate, the chromogenic reagent in Somogyi-Nelson method, was replaced by Folin-Ciocalteu phenol reagent. The major object was to remove the toxic arsenic compounds from the color reaction system. The color-producing ability of the phenol reagent was considerably lower than that of Nelson's reagent. However, the modified method was favorably comparable to Somogyi-Nelson method in simplicity, reproducibility and stability of color development. The error in both the modified and Somogyi-Nelson method could be reduced to about one fourth by adding sodium benzoate (final concentration, about 0.5%) to the test solutions.