Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Screening of L-Alloisocitrate-Utilizing Microorganisms
Teruhiko BEPPUKatsuya GOMIDalel Singh DAHIYAShigeru HOSHIKOKei ARIMA
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1980 年 44 巻 4 号 p. 759-764

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Microorganisms utilizing erythro-LS isocitrate (L-alloisocitrate) were isolated from soil after enrichment culture in medium containing the acid as the sole carbon source. Most isolates were bacteria, and NAD+-linked L-alloisocitrate dehydrogenase activity was found in some of their cell-free extracts. A bacterial strain No. 2 showing the highest enzyme activity was identified as belonging to genus Pseudomonas. A fungal strain showing good growth on L-alloisocitrate but restricted growth on glucose was also isolated and identified as belonging to genus Acremonium. No dehydrogenase nor oxidase activity was detected for L-alloisocitrate in extracts of the fungus, although conversion of the acid to fumarate was suggested in tracer
experiments with intact cells.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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