Simulation of the Process of PL-l Phage Adsorption to Its Host Bacterium, Lactobacillus casei ATTCC 27092

抄録

A kinetic model representing PL-1 phage adsorption to host bacterium Lactobacillus casei ATCC 27092 was simulated by using a digital computer. By trial and error process, equation (I) agreed closely with the experimental results, and the approximate values of the parameter, rate constant, were calculated to be: k₁B=0.19, k₂=0.04, k₃=0.04 and k₄B=0.04 (1/ml).
Reversible complex (b)_??_Free phage_??_Reversible complex (a)_??_Irreversible complex
(1) In this case, however, for a best fit it was necessary to make an additional assumption that the phage population contained a small proportion of slowly-adsorbing phages and to make some corrections for k₁ and k₄ after the relative amount of free phage against input phage taken as 1.0 decreased below 0.1. The existence of such heterogeneous phages was confirmed experimentally. The significance of the parameter in the process of PL-1 phage adsorption was discussed.