Isolation of a New Tetracycline Resistance Gene Transducing Bacteriophage Lambda and Its Properties

抄録

Six tetracycline resistance (Tc^R) gene transducing lambda phages were obtained from a lysate of Escherichia coli strain C600 (λb₁₅₁b₅₁₉) (Rl00-1). This strain was previously prepared by the following steps: cells were lysogenized with λb₅₁₅b₅₁₉ and multi-drug resistance factor R100-1 was introduced by mating. Each DNA extracted from six lambda phages called λptet, was characterized by the integration site of Tc^R gene in lambda whole genome. The site was determined by analysis of cleavage patterns of restriction endonuclease, EcoRI and HindIII and of heteroduplex figures under the electron microscope. The results indicated that three λptet phages, λptet 36, 41 and 105, accepted Tc^R gene at λ₂ region. The other three λptet phages, λptet 1, 27 and 115, accepted it at or near Q gene.