Derepressed Syntheses of Speculation Marker Enzymes in a Bacillus Species Mutant

Abstract

Sporulation marker enzymes, D-glucose dehydrogenase and L-alanine dehydrogenase were synthesized derepressively by vegetative cells of a Bacillus species mutant which was isolated as an improved D-ribose producer. It was also elucidated by electron microscopy that no morphological change concerning sporulation took place during the course of the enzyme syntheses in the mutant strain. The presence of Mn²⁺ and Ca²⁺ in the mediumwas necessary for the morphological development of sporulation even in the mutant strain. The mechanismof derepressed enzyme syntheses is discussed in relation to regulation of sporulation.