L-Arginine Production by Arginine Analog-resistant Mutants of Microorganisms

Abstract

L-Arginine analog-resistant mutants were derived from Bacillus subtilis, Serratia marcescens, Microbacterium ammoniaphilum, Micrococcus sodonensis, Nocardia corynebacteroides, N. rubra, Saccharomyces cerevisiae and Candida tropicalis. The mutants of all species tested produced an appreciable amount of L-arginine. The arginine productivity of SAH4-7, an L-arginine hydroxamate-resistant L-arginine-producer of B. subtilis, increased stepwisely by successively introducing such characters as pyrimidine analog-, histidine analog-, and tryptophan analogresistance and then increased resistance to arginine analog. The mutant strain finally selected was KY7690 and it produced ca. 17mg per ml of L-arginine.