Microbial Production of Plant Gibberellins and Related Compounds from ent-Kaurene Derivatives in Gibberella fujikuroi

Abstract

ent-15α-Hydroxykaurenoic acid (8) was synthesized and fed to a myceliumsuspension of Gibberella fujikuroi in the presence of 1-n-decylimidazole, a gibberellin biosynthesis inhibitor. The metabolites included 15β-hydroxy GA₂₄, GA₄₅ (GA of Pyrus communis), 15β-hydroxy GA₁₅ and 15β-hydroxy GA₂₅. Microbial production of 12α-hydroxy GAs from ent-12β-hydroxykaurene is also described.