The Synthesis of New Polymer Derivatives of ATP by Radical Copolymerization and Their Coenzymic Activity

Abstract

Three polymerizable ATP derivatives, N⁶-[N-(6-methacrylamidohexyl)carbamoylmethyl]-, N⁶-[N-[2-[N-(2-methacrylamidoethyl)carbamoyl]ethyl]carbamoylmethyl]-, and N⁶-[N-[N-(2-hydroxy-3-methacrylamidopropyl)carbamoylmethyl]carbamoylmethyl]-ATP, were synthesized and radically copolymerized with comonomers [acrylamide, N-(2-hydroxyethyl)-, N-ethyl-, N, N-diethylacrylamide, acrylic acid, and 6-methacrylamido hexylammonium chloride] to obtain 18 new polymer derivatives of ATP. The molecular weight distributions were controlled by appropriate initiator concentrations. The monomeric and polymeric ATP derivatives were all coenzymically active against both hexokinase and glycerol kinase. The observed coenzymic activities (Km and V_max) are discussed in connection with the structures of the derivatives.