Localization of Allergenic Reactive Sites on Hen Ovomucoid

Abstract

Our previous work on the allergenic structure of ovomucoid has shown that the allergenic reactive sites reside within the two fragments (domain I-II and domain II-III) comprising two-thirds of ovomucoid. In the present study, domain II was isolated and its allergenic reactivity was determined with a passive cutaneous anaphylaxis reaction using specific IgE antibody from mouse. The reactivity was estimated to be 40% as compared with the total reactivity in ovomucoid. The allergenic reactive sites still reside within domain II but the combination of domain II with either domain I or domain III is essential for full exhibiting of the allergenic reactivity in ovomucoid.