Affinity Chromatography of Neutral Proteinase from Streptomyces naraensis on a Column of N-Carbobenzoxy-L-phenylalanyl-aminohexyl-Sepharose

Abstract

N-Carbobenzoxy-L-phenylalanyl-aminohexyl-Sepharose (Z-Phe-AH-Sepharose) was found to be an effective affinity adsorbent for neutral proteinase from Streptomyces naraensis. This enzyme was adsorbed on this affinity column at pH 7.0 or higher, and eluted with 0.3 M NaCl in starting buffer. The resulting enzyme appeared homogeneous on disc electrophoresis and showed considerably higher specific activity and recovery than by previous method.