Abstract
Washed cells of facultative methylotrophs which have the serine pathway showed high activities for L-methionine formation from DL-homocysteine, in the presence of methanol as methyl donor. Strain FM 518, isolated from soil and identified as a bacterium belonging to the genus Pseudomonas, showed the highest activity for L-methionine formation and was used as the parental strain for breeding the L-methionine-producing mutants. An ethionine-resistant mutant, FE 244, derived from strain FM 518, accumulated 0.8 mg/ml L-methionine in a methanol-medium under optimum conditions.
