A Computer Simulation of the Adsorption of Lactobacillus Phage PL-1 to Host Cells: Some Factors Affecting the Process

抄録

A computer simulation of the kinetics of the adsorption of PL-1 phages to cells of Lactobacillus casei ATCC 27092 was done using the model formulated as in equation (1).
Reversible complex (b) K₄B K₂ Free phage K₁B K₂ Reversible complex (a) K₃ Irreversible complex
Under optimum conditions, the approximate values for the parameters were computed to be: k₁B=0.2, k₂=0.06, k₃=0.04 and k₄B=0.07 (1/ml). When the cell concentration, reaction temperature or medium was changed, all computed curves fitted the experimental data fairly well by changing the values for the parameters in the same model. Thus, the validity of the modified sequential model proposed for PL-1 phage adsorption in equation (1) was partly confirmed.