Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Growth of Myeloma MPC-11 Cells in Serum-free Growth Factor Supplemented Medium
Hiroki MURAKAMI, Toshikazu EDAMOTO, Hirofumi NAKAMURA, Hirohisa OMURA
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1982 年 46 巻 7 号 p. 1831-1837

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A myeloma MPC-11 cell line, which originated in a mouse plasma cell tumor, was able to proliferate in the absence of serum in a synthetic medium supplemented with transferrin, ethanolamine and selenium. The cells showed almost the same growth rate in this medium as in medium with 10% fetal calf serum added. Immunoglobulins secreted into the serum-free medium by the cells were easily separated from medium components by polyacrylamide gel electrophoresis.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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