Influence of Pyrophosphate on the Interaction of Leuconostoc Phosphoglycerate Mutase with Cibacron Blue

Abstract

Phosphoglycerate mutase (EC 2.7.5.3) of Leuconostoc dextranicum AHU 1078 adsorbed onto blue dextran-Sepharose, was easily eluted with a low concentration of pyrophosphate. Pyrophosphate inhibited the mutase competitively with respect to 3-phosphoglycerate, but noncompetitively with respect to 2, 3-bisphosphoglycerate. Further inhibition studies suggested that two molecules of pyrophosphate bound per active site of the phosphoglycerate mutase. The inactivation of the Leuconostoc mutase by pyridoxal phosphate was prevented by the addition of pyrophosphate. Spectral measurements revealed that the mutase-Cibacron Blue complex in solution was dissociated by the addition of pyrophosphate.