Extracellular Production of D-(+)-2-Hydroxyglutaric Acid by Yarrowia lipolytica from Glucose under Aerobic, Thiamine-deficient Conditions

Abstract

Under thiamine-deficient, aerobic culture conditions, Yarrowia lipolytica was found to produce D-(+)-2-hydroxyglutaric acid extracellularly in amounts of about 5mg per ml of the culture filtrate, together with pyruvic and 2-ketoglutaric acids, from glucose or glycerol in a chemically defined medium. Under similar conditions, however, only a small amount of the hydroxy acid was produced from odd- or even-numbered n-alkanes.