Abstract
Ovomucoid domains I (Ala1 … Met68) and III (Leu131 …Cys186) were prepared from chicken egg white ovomucoid by CNBrcleavage and Staphylococcus aureus V8 protease digestion, and antigenic properties of each domain were investigated by immunodiffusion, immunoprecipitation and the rat passive cutaneous anaphylaxis (PCA) test. Both domains I and III formed a precipitin arc or immunoprecipitation against rabbit anti-ovomucoid serum, and inhibited the PCAreaction of mouseanti-ovomucoid serum. These results indicated that ovomucoidantigenic sites existed in both domains I and III, and that the domains retained their antigenicity even after they were separated.
