抄録
Thermomonospora curvata IFO 12384 was selected from many thermophiles tested as a strain capable of converting 2, 6, 6-trimethyl-2-cyclohexene- 1, 4-dione (4-oxoisophorone) into (6R)-2, 2, 6- trimethyl-1, 4-cyclohexanedione ((3R)-dihydro-4-oxoisophorone) efficiently. The other conversion products were also isolated and identified by spectrometry. Based on the results of experiments with a degradation sequence, a conversion pathway for 4-oxoisophorone by this thermophile is proposed. The effects of nutritional conditions, temperature, initial pH and the concentration of the substrate on the conversion were examined. Under the optimal conditions, the conversion ratio of 4-oxoisophorone to (3R)-dihydro-4-oxoisophorone was over 95%. The production rate of (3R)- dihydro-4-oxoisophorone was 86mg per 1 g of dry cells per hr.
