Cloning and Expression of a Gene for Resistance to Butirosin both in Escherichia coli and Bacillus subtilis

抄録

We have cloned a butirosin resistance gene located on a 2.9Md HindIII fragment of the chromosomal DNA of Bacillus vitellinus Z-1159, a butirosin producer, on a shuttle vector, pTA1302 (5.2Md, Ap^r, Cm^r). The recombinant plasmid conferred resistances to butirosin, neomycin and kanamycin, on both Escherichia coli and Bacillus subtilis. Functional expression of aminoglycoside-B '-phosphotransferase activity from the butirosin resistance gene was confirmed in the cell extracts of both E. coli and B. subtilis harboring the recombinant plasmid. A cleavage map of the recombinant plasmid was constructed.