An Arabinoglucuronomannan from Extracellular Polysaccharides of Suspension-cultured Tobacco Cells

Abstract

A polysaccharide containing L-arabinose, D-mannose, D-galactose, and D-glucuronic acid residues in the molar ratio of 46.9:26.0: 3.9:23.2 was isolated from the extracellular polysaccharides of suspension-cultured tobacco cells by ion-exchange chromatography and barium hydroxide precipitation. Structural studies were performed by methylation analysis, ¹³C-NMR spectroscopy, partial hydrolysis with acid, and carboxyl-reduction. From those experiments, a structure for the polysaccharide is proposed.