Abstract
Regenerating protoplasts of Pyricularia oryzae P2 accumulate electron-transparent granules of polysaccharide in the cytoplasm. The granules were isolated from regenerating protoplasts and purified by repeated ultracentrifugation. The polysaccharide had a molecular weight of over 2×107 and a sedimentation constant of 160 S. The polysaccharide was composed of glucose only. Conversion into maltose with α-amylase, β-amylase and isoamylase was 52, 32 and 13%, respectively. However, the simultaneous action of β-amylase and isoamylase brought about complete hydrolysis of the polysaccharide to maltose. A highly branched structure with an average chain length of 14 glucose units was deduced. The absorption maximumof the iodine complex was at 490nm. From these results, the polysaccharide was identified as glycogen.
