Abstract
The enzymatic preparation of laminaribiose was investigated in this research. Whencurdlan was hydrolyzed with the β-l, 3-glucanase system of Streptomyces sp. K27-4, the hydrolyzate mainly consisted of glucose and laminaribiose in an approximate ratio of 1 : 1 by weight. Yeast strains selected in this study, effectively and selectively metabolized all of the glucose in the hydrolyzate, without any degradation of laminaribiose.
By successive treatment of curdlan with the glucanase system and glucose-metabolizable yeast, 31g of crystalline laminaribiose was obtained from 1OOg of curdlan.
