Abstract
The endogenous gibberellins in rather matured seeds of Pharbitis purpurea were qualitatively analyzed by combined gas chromatography/mass spectrometry, and gibberellins A3, 5, 8, 17, 19, 20, 26, 27, 29, 34, 44 and 53 were identified. A semiquantitative analysis of these gibberellins was performed on a small quantity of very young, immature seeds by gas chromatography/selected ion monitoring (GC/SIM) after purification by high performance liquid chromatography. The mass spectra of two types of these gibberellin derivatives, methyl ester-trimethylsilyl ethers and trimethylsilyl ester-trimethylsilyl ethers, were measured and their advantages and disadvantages in GC/SIM analysis are discussed.
