Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Qualitative and Semi-quantitative Analyses of Gibberellins in Immature Seeds of Pharbitis purpurea
Sachiko FUJISAWAIsomaro YAMAGUCHIKeun-Hyung PARKMasatomo KOBAYASHINobutaka TAKAHASHI
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1985 年 49 巻 1 号 p. 27-33

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The endogenous gibberellins in rather matured seeds of Pharbitis purpurea were qualitatively analyzed by combined gas chromatography/mass spectrometry, and gibberellins A3, 5, 8, 17, 19, 20, 26, 27, 29, 34, 44 and 53 were identified. A semiquantitative analysis of these gibberellins was performed on a small quantity of very young, immature seeds by gas chromatography/selected ion monitoring (GC/SIM) after purification by high performance liquid chromatography. The mass spectra of two types of these gibberellin derivatives, methyl ester-trimethylsilyl ethers and trimethylsilyl ester-trimethylsilyl ethers, were measured and their advantages and disadvantages in GC/SIM analysis are discussed.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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