Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Changes in Surface Charge, Respiratory Rate and Stainability with Crystal Violet of Resting Escherichia coli B Cells Anaerobically Exposed to an Alternating Current
Keiko SHIMADAKenzo SHIMAHARA
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1985 年 49 巻 2 号 p. 405-411

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Phosphate buffer suspensions of resting Escherichia coli B cells at pH 7.0 were anaerobically exposed to an alternating current (AC) of 50 Hz at a current density of 600 mA/cm2 and 34±3°C. The AC-exposed cells required a higher concentration of a cationic flocculant, poly(methacryloxyethyl-trimethyl-ammonium chloride), to form cell floes than unexposed cells. The electrophoretic mobility of the cells increased after AC-exposure. Oxygen uptake by the exposed cells in a neutral reaction mixture decreased with increasing exposure time. The cells were less easily stained with crystal violet after AC-exposure. These observations show that AC-exposure causes an increase in the amount of the negative surface charge of E. coli cells and changes in some physiological properties.
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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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