抄録
Cultural conditions for the microbial production from gallic acid of pyrogallol were investigated with Citrobacter sp. The maximal formation of pyrogallol was observed when the bacterium was cultured at 28°C for 48 hr in a medium containing 2.0% gallic acid, 0.5% maltose, 0.2% (NH4)2HPO4, 0.1% KH2PO4, 0.05% KC1, 0.05% MgSO4•7H2O and 0.05% yeast extract (pH 6.0). Under these conditions, 7.2 g of pyrogallol was formed from 20 g of gallic acid added to one liter of the medium. Larger amounts of pyrogallol could be produced when gallic acid was further added to the broth after 16-24hr cultivation. The reaction was performed at pH 4.5 under anaerobic conditions using 1 mM L-ascorbic acid and N2 gas. Seventy-five grams of gallic acid added to one liter of the broth was transformed to 54.1 g of pyrogallol under these conditions, with a yield of 97.4%.
