1985 Volume 49 Issue 4 Pages 981-985
Laccase from Polyporus versicolor was found to develop a strong blue or red color with phenol in the presence of coupling compounds such as 2, 6-dibromo-4-aminophenol or 4-aminoantipyrine. Based on this phenomenon, concentrations of phenol (0.05-0.4 HIM) could be determined from the density of the color developed with laccase. Thus, assay methods for α-glucosidase and α-amylase were designed. α-Glucosidase (0.02-0.2 units) hydrolyzed phenyl α-glucoside and the phenol released was converted to a colored substance by laccase. Human α-amylase could also be determined using phenyl maltotetraoside or pheny maltopentaoside as the substrate. The phenyl maltooligosaccharide was hydrolyzed to phenol and glucose by the combined action of α-amylase and α-glucosidase, and the resultant phenol was assayed by the same method as described above. The proposed method may be used in conjunction with a conventional photometer, because the color developed is reddish or blue.
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