Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
Development of a Host-vector System for Gluconobacter suboxydans
Masahiro FUKAYAHajime OKUMURAHiroshi MASAITakeshi UOZUMITeruhiko BEPPU
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JOURNALS FREE ACCESS

1985 Volume 49 Issue 8 Pages 2407-2411

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Abstract

A shuttle vector for Gluconobacter suboxydans and Escherichia coli was constructed by ligation of a cryptic plasmid, pMV201, found in G. suboxydans IFO 3130 to E. coli plasmid pACYC177. The chimeric plasmid named pMG101 carries the ampicillin resistance gene derived from pACYC177 and transforms G. suboxydans var. α IFO 3254 as well as E. coli. The transformation conditions for G. suboxydans var. α IFO 3254 were examined using pMG101 DNA. Competent cells were induced efficiently by treatment with LiCl or RbCl. CaCl2 which induced the competency of Acetobacter was much less effective. Addition of polyethylene glycol enhanced the transformation efficiency significantly. An efficiency of approximately 102 transformants per μg DNA was finally obtained.

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