Synthesis of Enzymatically Active Mouse Submandibular Gland Renin in Escherichia coli
1986 年 50 巻 2 号 p. 271-279
詳細
1986 年 50 巻 2 号 p. 271-279
A plasmid (pMR226) containing the cDNA coding for the complete amino acid sequence of mouse renin was constructed. The cDNA was reconstructed so as to code for either fused mature renin or preprorenin, and inserted into a plasmid designed to express a protein under the control of the Escherichia coli tryptophan promoter. When introduced into E. coli, the resultant plasmids (pMR304, pMR324) directed the synthesis of proteins that cross-reacted with an antiserum raised against purified mouse renin. The renin precursor produced was readily activated on trypsin treatment and the activity was inhibited by pepstatin and antirenin-antibody.
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