Microbial Dehalogenation of Haloalkanes Mediated by Oxygenase or Halidohydrolase

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Microorganisms utilizing 1-chlorobutane as a sole carbon and energy source for growth could release halogens under anaerobic conditions, while microorganisms which could utilize 1, 9-dichlorononane released the halogens only under aerobic conditions. A 1-chlorobutane-utilizing bacterium, strain m15-3, converted 1-chlorobutane to butyric acid and 1, 3-dichloropropane to 3-chloropropionic acid under aerobic conditions and 1-chlorobutane to butanol under anaerobic conditions. In the latter case, the participation of halidohydrolase was suggested.
Methane-utilizing bacteria catalyzed the removal of halogens from the terminal positions of short chained chlorinated hydrocarbons. Methane-utilizing bacteria dehalogenated 1, 2-dichloroethane to ¹⁸O-incorporated 2-chloroacetic acid in the presence of ¹⁸O₂ gas.
All of the seven bacterial strains used in this study dehalogenated 3-chlorinated aliphatic acids, but only one strain out of seven could dehalogenate 2-chlorinated aliphatic acids.