Construction of an Expression and Secretion Vector for the Yeast Saccharomyces cerevisiae

Abstract

In order to make yeast cells secrete useful materials, an expression and secretion vector was constructed using the promoter and the signal sequence of yeast invertase coded by the SUC2 gene. When mouse amylase, after removal of its own signal sequence, was ligated with the secretion vector pSMF38T, amylase activity was secreted by the yeast Saccharomyces cerevisiae. The amount of the secreted enzyme was estimated to be 600μg/liters culture under the derepression conditions for the SUC2 promoter function.