Abstract
We have previously constructed hEGF secretion vector pTA1522 in which phoA signal peptide-hEGF hybrid gene was expressed under the control of the phoA promoter, and have shown that Escherichia coli carrying pTA1522 produced authentic hEGF in large amounts. The present report describes the relative efficacies of a number of secretion vectors that utilized various promoters (phoA, trp, rightward and leftward promoter of phage λ), signal peptides (phoA, bla), and antibotic resistance markers (Ampr, Kmr) in different combinations. The most effective was pTA2532 with phoA promoter, a bla signal peptide coding region, and Kmr gene. The amount of hEGF secreted was 2.12×105 molecules per cell.
