抄録
A putative structural gene (RHA). coding for rhodotorucine A, a farnesyl peptide mating pheromone in cells of mating type A of Rhodosporidium toruloides, has been cloned using synthetic oligonucleotides corresponding to the amino acid sequence of rhodotorucine A. The RHA gene contains sequences for four tandem copies of the rhodotorucine A peptide, each followed by a spacer peptide of three or four amino acids. The spacer peptides are hypothesized to contain signals for proteolytic processing and farnesylation. The results of Southern blot analysis revealed that the RHA gene is specifically contained in the genome of A-type cells, and not in that of complementary a-type cells. The existence of an additional homologous gene in the genome of A-type cells is also suggested.
