Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Enzymatic Reduction of Hexavalent Chromium by Hexavalent Chromium Tolerant Pseudomonas ambigua G-1
Hiroyuki HORITSUSatoshi FUTOYoshimi MIYAZAWAShusuke OGAIKeiichi KAWAI
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1987 Volume 51 Issue 9 Pages 2417-2420


When hexavalent chromium (Cr6+) tolerant Pseudomonas ambigua G-1 was cultivated in nutrient broth containing 150ppm Cr6+, the Cr6+ content of the broth rapidly decreased. The Cr6+ reducing enzyme found in a cell-free extract of P. ambigua G-1 required NADH but not NADPH as a hydrogen donor for the reduction of Cr6+. The specific activities of cell-free extracts of several Cr6+ sensitive mutants derived from P. ambigua G-1 showed decreases to one fourth to one tenth of that of P. ambigua G-1. Glucose protected the Cr6+ reducing enzyme against inactivation on dialysis.

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