Abstract
The complete amino acid sequence of metallothionein II isolated from kidneys of the striped dolphin, Stenella coeruleoalba, is reported. The sequence was established by a gas phase protein sequencer using the fragments obtained by cyanogen bromide cleavage and protease digestions of the S-carboxamidomethylated protein. The N-terminal was identified as acetylmethionine by 1H-NMR and MS using the N-terminal dipeptide obtained by formic acid hydrolysis. The C-terminal amino acid was assigned as alanine using both carboxypeptidase and trypsin digests. Remarkable sequence homology was observed between dolphin and mammalian metallothionein II,
