Cloning and Nucleotide Sequences of the Two 130kDa Insecticidal Protein Genes of Bacillus thuringiensis var. israelensis

Abstract

Two types of 130kDa insecticidal protein genes isolated from large plasmid DNAs of Bacillus thuringiensis var. israelensis HD522 were cloned in an Escherichia coli plasmid vector. Analysis of the nucleotide sequences revealed that the two genes encoded a 127, 500-dalton protein (ISRH3) consisting of 1, 135 amino acids and a 134, 400-dalton protein (ISRH4) consisting of 1, 180 amino acids. The two insecticidal proteins were identical in a region of the C-terminal 467 amino acids.