Isolation of Advantageous Mutants of Pseudomonas chlororaphis B23 for the Enzymatic Production of Acrylamide

Abstract

Advantageous mutants of Pseudomons chlororaphis B23 for the enzymatic production of acrylamide were isolated. A mucilage polysaccharide non-producing mutant, Am-3, was precipitated completely by brief centrifugation, in contrast with the parent strain. A mutant, AM-324, derived from Am-3 exhibited about 3.8-fold higher nitrile hydratase activity than that of the parent strain. These mutants are promising for acrylamide production on an industrial scale.