The Amino Acid Sequence of Indian Peafowl (Pavo cristatus) Lysozyme and Its Comparison with Lysozymes from Phasianoid Birds

Abstract

The amino acid sequence of Indian peafowl egg-white lysozyme has been identified. The reduced and carboxy methylated lysozyme was digested with trypsin followed by purification of the resulting peptides by reverse-phase HPLC. The tryptic peptides obtained were sequenced using the DABITC/PITC double coupling manual sequencing method. The alignment of the tryptic peptides were deduced by comparison with corresponding peptides of hen egg-white lysozyme. This protein proved to consist of 129 amino acid residues, and a relative molecular mass of 14423 Da was calculated. Amino acid sequence comparison of peafowl lysozyme and other phasianoid bird lysozymes revealed a maximum homology ratio of 98% with turkey lysozyme.