Abstract
Interspecific protoplast fusion between Candida utilis IAM 4277 and Candida lipolytica OUT 6340 was carried out. A stable fusant, No. 1, was selected from among many fusants which were able to grow on both maltose and n-hexadecane. Fusant No. 1 seems to be an intermediate between the parents with respect to morphological and physiological characteristics. Further chemotaxonomic studies on the coenzyme Q type, fatty acid composition and enzyme patterns, and antigenic analysis revealed that fusant No. 1 is a hybrid having both a major part of the characteristics of C. lipolytica OUT 6340 and a minor part of those of C. utilis IAM 4277.
